- Factual errors: All the flags shown hanging from various buildings in New York City are in perfect condition. These flags would have been torn and faded from three years of hanging in the weather.
- Factual errors: Robert Neville is a Lieutenant Colonel, but his insignia in the shots portraying him in his military dress uniform are golden, which indicate the rank of Major.
- Continuity: The bite marks on the right shoulder of Neville jump to his left shoulder during a cut shot in the final scene in the basement lab.
- Revealing mistakes: During the beginning scenes, where the camera pans over the city, during one pass over a road that runs horizontally across the picture, if you look closely people can be seen walking close to the buildings.
- Factual errors: In the final battle scene, during the initial invasion of the apartment, the M-4 depicted runs out of ammo, and is depicted as beginning to "click click click". This is not how the M-16 (M4) class of weapons work. The action is gas-driven based on the last round fired. The trigger/firing pin does not continue working once the ammo source is depleted. This sound would indicate an electric-driven firing system.
- Continuity: In one scene, you can see Vincent van Gogh's "Starry Night" hanging on the wall as they run up the stairs. In a later scene, it's hanging over the fireplace.
- Continuity: If you look out of the car window on the driver's side (at roughly the 3:18 mark) just after Neville grabs his gun, you will see a silver and red car parked (silver on left, red on right). Neville continues driving down the road and away from the parked cars, but at around the 4:00 mark, when you get another clear shot out his window (over his shoulder), you can see the same two cars parked, yet he is not in the same area where they first appeared.
- Revealing mistakes: In the beginning of the movie, Neville is seen pouring vinegar. Just like one of those trick baby bottles that never run out, it appears to get low and fill up on its own.
- Continuity: When Neville is on the carrier and raises his gun to look at the deer, the flashlight on the gun is on the right side of the screen. When Neville goes inside the building to look for Sam, the flashlight on his gun is on the left side of the screen.
- Factual errors: The hand grenade Neville pulls out in the end scene has a blue arming spoon. Only dummy grenades have blue arming spoons, meaning the grenade isn't a real one.
- Factual errors: Lions do not kill by biting the back of the prey's neck, as shown. They strangle their prey by biting the windpipe, like all great cats. Only clumsy, inexperienced cats bite the back of the neck, and the one shown is obviously a skilled hunter.
- Factual errors: A running man can't approach a fleeing whitetail deer's speed.
- Continuity: After his first swing, we can clearly see only 3 balls remain on the YF-12A, and they're immediately beside the dog. But when the shot reverses, he hits another without resetting a ball, or taking a step forward, or disturbing the dog.
- Factual errors: When Neville opens the drawer in his lab and grabs the grenade, it is shown as a fragmentary grenade. However, when he sets it off, it explodes in a burst of flame. Fragmentary grenades explode without large flames and throw shrapnel. The explosion shown would have been from an incendiary grenade, which looks like an aerosol canister and not the rounded frag grenade.
- Factual errors: When Neville fires his gun (an M4A1 carbine) at the dark seeker in the house and runs out of ammo, the weapon can be heard clicking several times. An M4A1 will not click when the magazine runs empty. The bolt carrier group will lock to the rear holding the hammer down; pulling the trigger has no further effect.
>>> WARNING: Here Be Spoilers <<<
Goofs below here contain information that may give away important plot points. You may not want to read any further if you've not already seen this title.
- Crew or equipment visible: SPOILER: When Neville is in the dark warehouse looking for Sam, he enters a bathroom. When his flashlight hits a mirror, he ducks and shields the light, but there is still a circle of light from the camera behind him.
- Audio/visual unsynchronized: SPOILER: Neville states the lab doors are made of Plexiglas (acrylic), but when the "alpha" Dark Seeker smashes them, the sound is that of breaking glass.
- Continuity: SPOILER: During the scene on the dock just before Neville's rescue by Anna and Ethan: the driver's side window is shattered several times, but exterior views of the SUV shows the window intact.
- Continuity: SPOILER: In the opening scene, Neville's M4 rifle has a sling attached. After the deer he is aiming at gets attacked by the lion, the rifle no longer has a sling.
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